FastQCFastQC Report
Sat 31 Dec 2022
SRR1953487.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953487.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1446199
Sequences flagged as poor quality0
Sequence length86
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA45030.31136793760748No Hit
GAGCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTC41470.28675168493409275No Hit
GAGAGCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCT34710.2400084635655259No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30920.2138018350171726No Hit
AAGAGCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCT26790.18524421604495647No Hit
TAGAGCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCT24630.17030851217571027No Hit
AAGCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTC23310.1611811375889487No Hit
GCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTG20260.14009137055135565TruSeq Adapter, Index 21 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)