FastQCFastQC Report
Sat 31 Dec 2022
SRR1953481.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953481.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1572782
Sequences flagged as poor quality0
Sequence length86
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA41010.2607481520007223No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36680.23321731810257237No Hit
GAGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTC30630.1947504485682059No Hit
GAGAGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCT28740.18273352568887488No Hit
AAGAGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCT20990.13345778372336406No Hit
AAGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTC20940.13313987571068336No Hit
TAGAGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCT19730.12544650180381006No Hit
GCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTG16750.10649918424803946TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)