FastQCFastQC Report
Sat 31 Dec 2022
SRR1953479.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953479.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1215605
Sequences flagged as poor quality0
Sequence length86
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAGCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCT34320.28232855244919197No Hit
GAGCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTC33430.2750070952324151RNA PCR Primer, Index 33 (95% over 21bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA32040.26357245980396593No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29700.2443227857733392No Hit
AAGAGCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCT23970.19718576346757377No Hit
AAGCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTC22680.18657376368145903RNA PCR Primer, Index 33 (95% over 21bp)
TAGAGCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCT21430.1762908181522781No Hit
GCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTG13360.10990412181588591RNA PCR Primer, Index 33 (95% over 23bp)
TGTTTAGGGGTTATATTTCAACTGGTCTGGAAATGTGCTGTTTACAGAAT12910.10620226142538076No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)