FastQCFastQC Report
Sat 31 Dec 2022
SRR1953477.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953477.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2481803
Sequences flagged as poor quality0
Sequence length86
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92070.37098029134463933No Hit
GAGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTC50950.20529429612261732No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG49950.20126496744503897No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43110.17370435929040298No Hit
AAGAGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCT40850.1645980764790759No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC40020.16125373367668586No Hit
AAGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTC39190.1579093908742958No Hit
TAGAGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCT36850.14848076176876246No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA36550.14727196316548896No Hit
GAGAGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCT36410.146707857150628No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG28590.11519850689196524No Hit
TCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG26660.10742190254423901No Hit
AGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT25050.10093468337333784No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)