FastQCFastQC Report
Sat 31 Dec 2022
SRR1953474.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953474.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2638106
Sequences flagged as poor quality0
Sequence length86
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103120.3908864920514945No Hit
GAGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTC50860.19278982724727514No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG49280.18680068200443803No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42920.16269247710289125No Hit
GAGAGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCT42800.16223760531229603No Hit
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC39640.15025931482662183No Hit
AAGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTC37200.14101025508451898No Hit
TAGAGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCT36680.13903914399193967No Hit
AAGAGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCT33820.12819803298275353No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA33750.12793269110490632No Hit
GATTATGGGACTTCTTGCTTTTCATGATGCATGTTTCTGTATGTTAATGA27640.10477213576709958No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)