FastQCFastQC Report
Sat 31 Dec 2022
SRR1953473.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953473.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1644813
Sequences flagged as poor quality0
Sequence length86
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66410.40375410456994204No Hit
GAGAGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCT31710.19278787315032164No Hit
GAGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTC30430.18500583348988608No Hit
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG27950.16992813164779216TruSeq Adapter, Index 22 (95% over 21bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA25140.15284412270574224No Hit
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC24920.15150658463910488No Hit
AAGAGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCT23830.1448796914907652No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22880.13910395893028568No Hit
TAGAGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCT22560.1371584490151768No Hit
AAGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTC21820.13265945733648749No Hit
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG16610.10098412403112086TruSeq Adapter, Index 22 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)