FastQCFastQC Report
Sat 31 Dec 2022
SRR1953470.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953470.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2823840
Sequences flagged as poor quality0
Sequence length86
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112360.39789789789789787No Hit
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG42660.15107088220295767TruSeq Adapter, Index 23 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41250.14607768145503994No Hit
GAGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTC40560.14363420023797382TruSeq Adapter, Index 23 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG38730.13715366309705934TruSeq Adapter, Index 23 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC37940.13435605416737492Illumina Single End Adapter 1 (95% over 21bp)
GAGAGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCT36470.12915037679188623No Hit
AAGAGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCT34510.12220947362456797No Hit
AAGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTC30790.109035922715168No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA30260.10715904583829111No Hit
TAGAGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCT30060.10645079041305458No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)