FastQCFastQC Report
Sat 31 Dec 2022
SRR1953468.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953468.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1253465
Sequences flagged as poor quality0
Sequence length86
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54910.4380656819296909No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA18980.1514202630308784No Hit
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG16950.1352251558679341RNA PCR Primer, Index 35 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16780.13386891536660378No Hit
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC15700.1252527992405053TruSeq Adapter, Index 10 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG15070.12022673150028122RNA PCR Primer, Index 10 (95% over 22bp)
GAGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTC12850.10251582612996774RNA PCR Primer, Index 35 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)