FastQCFastQC Report
Sat 31 Dec 2022
SRR1953462.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953462.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6445098
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT543820.8437730504640891No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCT77360.12002920669321086No Hit
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC76270.11833799889466382TruSeq Adapter, Index 27 (100% over 24bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTC72030.11175935571499455No Hit
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG64720.10041740249721572TruSeq Adapter, Index 27 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)