FastQCFastQC Report
Sat 31 Dec 2022
SRR1953459.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953459.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1471681
Sequences flagged as poor quality0
Sequence length86
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53960.36665554559717767No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA40040.27206983035046317No Hit
GAGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTC22990.15621591907485385No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20370.13841314795801535No Hit
AAGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTC19630.13338488436012969No Hit
AAGAGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCT18070.12278476109972203No Hit
TAGAGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCT16680.11333977947666647No Hit
GCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTG15530.1055255860475198No Hit
GAGAGCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCT15420.10477814145864492No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)