FastQCFastQC Report
Sat 31 Dec 2022
SRR1953457.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953457.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2101940
Sequences flagged as poor quality0
Sequence length86
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75220.35785988182345835No Hit
GAGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTC61080.29058869425387973No Hit
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG55760.2652787424950284RNA PCR Primer, Index 27 (95% over 22bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA52010.24743808101087567No Hit
AAGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTC45230.21518216504752752No Hit
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC38030.18092809499795426RNA PCR Primer, Index 38 (95% over 23bp)
GAGAGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCT36990.1759802848796826No Hit
AAGAGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCT36520.1737442553070021No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36330.1728403284584717No Hit
TAGAGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCT35550.16912947086976793No Hit
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG28390.13506570120935896RNA PCR Primer, Index 38 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG26790.12745368564278714RNA PCR Primer, Index 27 (95% over 22bp)
AGAGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTT23730.1128957058717185No Hit
TCTTCAGGGATTTCCCTTGCCCTCAGTTTTCCACTTTTGGGGTTTTTTAT22960.10923242338030581No Hit
AGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT21790.10366613699725015RNA PCR Primer, Index 38 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)