FastQCFastQC Report
Sat 31 Dec 2022
SRR1953452.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953452.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2036854
Sequences flagged as poor quality0
Sequence length86
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89340.4386175936026833No Hit
GAGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTC38430.18867331679148333No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA35720.17536848492822754No Hit
GAGAGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCT31880.1565158818452378No Hit
AAGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTC29120.14296557337933893No Hit
AAGAGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCT26700.1310845058114131No Hit
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTG26690.13103541049088446No Hit
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC25030.1228855872831337TruSeq Adapter, Index 14 (95% over 21bp)
TAGAGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCT23380.11478485939591153No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22630.1111027103562651No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)