FastQCFastQC Report
Sat 31 Dec 2022
SRR1953445.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953445.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1599683
Sequences flagged as poor quality0
Sequence length86
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71580.44746365373639657No Hit
GAGAGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCT19830.12396205998313416No Hit
GAGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC18210.11383505356998855No Hit
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG17220.1076463274286218Illumina PCR Primer Index 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC16970.10608351779696354TruSeq Adapter, Index 10 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16720.10452070816530525No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)