FastQCFastQC Report
Sat 31 Dec 2022
SRR1953444.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953444.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1470992
Sequences flagged as poor quality0
Sequence length86
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53340.3626124411281639No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA29970.20374006112881646No Hit
AAGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC23490.15968815601988318No Hit
AAGAGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCT23240.15798862264376692No Hit
GAGAGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCT23040.1566289959428739No Hit
GAGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC22270.15139443314443587No Hit
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG21010.14282878492880993No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20960.1424888782535867No Hit
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC19720.13405919270805008TruSeq Adapter, Index 16 (95% over 21bp)
TAGAGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCT16870.11468451222032477No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)