FastQCFastQC Report
Sat 31 Dec 2022
SRR1953440.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953440.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448435
Sequences flagged as poor quality0
Sequence length86
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16860.37597422145907433No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA11750.26202236667521495No Hit
GAGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTC9050.20181297177963362No Hit
GAGAGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCT8990.20047498522639848No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7460.16635632811890239No Hit
TAGAGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCT7190.16033538862934427No Hit
AAGAGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCT7140.15922039983498165No Hit
AAGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTC6720.1498544939623357No Hit
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTG6550.1460635320615028Illumina PCR Primer Index 5 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC4570.10190997580474316Illumina PCR Primer Index 5 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)