FastQCFastQC Report
Sat 31 Dec 2022
SRR1953438.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953438.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1669824
Sequences flagged as poor quality0
Sequence length86
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78900.47250488674255486No Hit
GAGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTC23860.14288931049020734No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22680.1358226974818903No Hit
GAGAGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCT22310.13360689509792648No Hit
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG20700.12396516040013797TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC18990.11372456019317005TruSeq Adapter, Index 22 (95% over 21bp)
AAGAGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCT17760.10635851443026331No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)