FastQCFastQC Report
Sat 31 Dec 2022
SRR1953436.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953436.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1699374
Sequences flagged as poor quality0
Sequence length86
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62330.36678212094571294No Hit
GAGAGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCT25710.1512910048052989No Hit
GAGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTC20470.1204561209009906No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18870.11104088917448425No Hit
AAGAGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCT17820.10486214335396445No Hit
CTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC17190.10115489586165259Illumina Single End Adapter 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)