FastQCFastQC Report
Sat 31 Dec 2022
SRR1953430.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953430.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1714827
Sequences flagged as poor quality0
Sequence length86
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68010.396599773621479No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27370.15960793712718543No Hit
GAGAGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCT25290.1474784336845641No Hit
GAGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTC24930.14537909655026426No Hit
TAGAGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCT22090.12881765915745438No Hit
AAGAGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCT19850.11575511698847754No Hit
CTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC18680.10893227130200306Illumina Single End Adapter 1 (95% over 21bp)
AAGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTC18340.10694956400849764No Hit
GCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG17340.10111807196877586No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)