FastQCFastQC Report
Sat 31 Dec 2022
SRR1953403.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953403.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442501
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCACTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC4860.10983026027059825RNA PCR Primer, Index 35 (95% over 23bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC4780.10802235475174068No Hit
AAGCTGACTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT4560.10305061457488232TruSeq Adapter, Index 10 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)