FastQCFastQC Report
Sat 31 Dec 2022
SRR1953307.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953307.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences777780
Sequences flagged as poor quality0
Sequence length51
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTATCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTC51990.6684409473115791No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCG48370.6218982231479339No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40180.5165985240042171No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGC35850.46092725449355854No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTC15730.2022422793077734No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCG15520.19954228702203708No Hit
GAATGAATGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCT13250.17035665612383963No Hit
GCTGTCACTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC12640.16251382138908174No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGC12410.15955668698089434No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11950.15364241816451954No Hit
GAATGAACTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTC10730.13795674869500374No Hit
GAACTGACTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCT8460.10877111779680629No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)