FastQCFastQC Report
Sat 31 Dec 2022
SRR1953275.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953275.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511773
Sequences flagged as poor quality0
Sequence length51
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42400.8284923198371152No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC14740.28801832062261984No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13280.2594900473452097No Hit
GCTGTCACTTATACACATCTGACGCTACGGTTGTCGTATGCCGTCTTCTGC8330.16276747698686722TruSeq Adapter, Index 22 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8190.16003188913834845No Hit
CTGTCTATTATACACATCTGACGCTACGGTTGTCGTATGCCGTCTTCTGCT7390.144399958575384TruSeq Adapter, Index 20 (95% over 22bp)
TTCGTTAGGTGACTGTATAAGAAAATACGTGGTAATTTTTTGGTCTTTGGA6550.12798643148427136No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6150.12017046620278914No Hit
CCTGTCACTTATACACATCTGACGCTACGGTTGTCGTATGCCGTCTTCTGC6000.11723947922223328TruSeq Adapter, Index 22 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)