FastQCFastQC Report
Sat 31 Dec 2022
SRR1953270.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953270.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417895
Sequences flagged as poor quality0
Sequence length51
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32590.7798609698608503No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCG18560.4441307026884744No Hit
GAATCTATCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTC17560.4202012467246557TruSeq Adapter, Index 19 (95% over 21bp)
GAATGAAACGGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGC14200.339798274686225No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC11130.26633484487730175No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9870.2361837303628902No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCG6450.15434499096663037No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5370.12850117852570622No Hit
GAATCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTC5270.12610823292932435RNA PCR Primer, Index 40 (95% over 22bp)
GAATGAATGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCT5180.12395458189258068No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGC4960.11869010158054055No Hit
GCTGTCACTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC4910.11749362878234963Illumina PCR Primer Index 8 (95% over 23bp)
AGGCGAAGGCACTTTCTTTATAAAGGAAAATATTTTCTTTTAATTACATCT4720.11294703214922408No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4610.11031479199320403No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)