FastQCFastQC Report
Sat 31 Dec 2022
SRR1953269.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953269.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511017
Sequences flagged as poor quality0
Sequence length51
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31060.6078075680456815No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCG21270.41622881430559056No Hit
GAATCTATCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTC19700.3855057659529918No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGC15250.2984245142529505No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9510.1860994839702006No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCG7560.14794028378703644No Hit
GCTGTCACTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTCTGC6550.1281757749742181TruSeq Adapter, Index 14 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGCCGTCTTC6120.1197611821133152No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCGTGTTTCGTATGC5780.11310778310701992No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC5530.10821557795533221No Hit
GATACTAAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT5520.10801988974926469No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5180.10136649074296941No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)