FastQCFastQC Report
Sat 31 Dec 2022
SRR1953260.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953260.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1155119
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42450.3674946044520088No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC26660.23079873155925926No Hit
GCTGTCACTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC15310.1325404568706774TruSeq Adapter, Index 23 (95% over 24bp)
CTGTCTATTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT14110.12215191681549692Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13730.1188622124646898No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12680.10977223991640687No Hit
CCTGTCACTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC12290.10639596439847322TruSeq Adapter, Index 23 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)