FastQCFastQC Report
Sat 31 Dec 2022
SRR1953256.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953256.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515751
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27340.5301007656795624No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCG23510.45584012440111604No Hit
GAATCTATCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTC22930.44459438760176906No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGC18460.35792465744128465No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC11640.22569030404206683No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8950.17353335233475067No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCG7430.14406176623991035No Hit
GAATGAATGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCT6460.12525424090307144No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGC6370.12350921277903486No Hit
GCTGTCACTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC6310.12234586069634378Illumina Single End Adapter 2 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6270.1215702926412164No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTC6230.12079472458608903No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)