FastQCFastQC Report
Sat 31 Dec 2022
SRR1953248.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953248.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences526141
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTATCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC44740.8503423987106119No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCG37560.7138770785777957No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGC33620.6389922093127127No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29600.5625868350879327No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCG12750.24233047795172777No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC12380.2352981425131286RNA PCR Primer, Index 16 (95% over 22bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC11050.21001974755816408No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGC11000.20906943195835337No Hit
GAATGAATGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCT9930.18873267812240446No Hit
GCTGTCACTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC9520.18094009020395674Illumina PCR Primer Index 7 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9050.17200712356573616No Hit
GAATGAACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTC7780.14786910733054448No Hit
GAACTGACTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCT7330.13931626693224822RNA PCR Primer, Index 16 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)