FastQCFastQC Report
Sat 31 Dec 2022
SRR1953214.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1953214.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1318818
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTATCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTC103090.7816848117026003No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCG75080.5692976589643151No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73540.5576205359647807No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGC62280.4722410522149379No Hit
GAATCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTC30630.23225342693229847No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCG24940.18910873221323943No Hit
GAATGAATGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCT23050.1747777176229017No Hit
GAACTGACTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT22810.1729579062463509No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22650.1717446986619837No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGC20950.15885436807808204No Hit
GCTGTCACTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC19780.14998278761739678TruSeq Adapter, Index 15 (95% over 21bp)
GAATGAACTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTC19250.14596403749418038No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)