FastQCFastQC Report
Sat 18 Jun 2016
SRR3137999_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137999_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4139292
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG248790.6010448163598993No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41050.039.3442961
GTATCAA88650.034.9223181
ATCAACG111300.027.4827863
CAACGCA113500.027.2047215
TCAACGC115150.026.7186534
TATCAAC117500.026.05692
AACGCAG120900.025.6563996
CTCGATA7050.023.50800565
ACGCAGA138250.022.0435897
TGCGACG5400.021.71654522
GCTCGAT7650.021.66161264
TGGTCGT7750.021.37562651
CGCAGAG142250.021.2790978
AGAGTAC143100.020.14572111
CGTACGA3700.019.58949360
GCTCGTC8650.019.54961439
GTACATG163400.019.4523351
CAGAGTA151000.019.34177410
GAGTACT66000.019.28009212
GCAGAGT154600.019.0668139
Produced by FastQC (version 0.11.5)