FastQCFastQC Report
Sat 18 Jun 2016
SRR3137999_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137999_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4139292
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG60830.14695749901190833TruSeq Adapter, Index 11 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40950.041.471521
GTATCAA87000.035.5490071
TGCGACG5900.029.82090222
TATGCCG25550.029.30202543
CTCGTAT24300.028.9632639
ATCAACG108650.027.7219543
TCTCGTA25400.027.5731238
CAACGCA109400.027.5003665
TATCAAC112050.027.46682
TCGTATG27800.027.17800940
CAATGCG6700.026.77445419
TCAACGC114100.026.5187644
GATCTCG26700.026.35950536
AACGCAG117650.025.805316
ATCTCGT27000.025.68359837
ATGCCGT29350.025.62578644
GCTCGAT7500.025.29995564
CTCGATA7700.025.09086465
CGTACTA30250.024.86306629
CGACGAG7150.024.60805124
Produced by FastQC (version 0.11.5)