FastQCFastQC Report
Sat 18 Jun 2016
SRR3137998_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137998_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3041203
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG105380.34650761557186416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27550.035.6364061
GTATCAA54400.032.295491
ATCAACG64400.026.9637073
CAACGCA67150.026.1759345
TCAACGC66950.025.9418414
TATCAAC69300.025.1508162
AACGCAG70300.025.1052326
CGACGAG4150.024.106624
TGCGACG3900.023.88201122
TATCGGC1602.246452E-923.681412
CTCGATA4850.023.48289965
CAATGCG4150.022.44074419
GCGCGAT2207.2759576E-1221.94817213
CGCGATA2207.2759576E-1221.94708614
TGATCGC4900.021.814089
ATGCGAC4500.021.46185121
ACGCAGA79550.021.4543237
CGTACGA3400.021.3156460
GCTCGAT5200.021.23896464
CGCAGAG81000.020.9858238
Produced by FastQC (version 0.11.5)