FastQCFastQC Report
Sat 18 Jun 2016
SRR3137998_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137998_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3041203
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23800.039.586491
GTATCAA51200.034.4438931
ATCAACG58800.029.4545233
CAACGCA59650.028.9186535
TCAACGC60900.028.3817334
TATCAAC64150.027.5367832
AACGCAG64200.027.0840726
CGTACGA3650.025.52051260
CAATGCG3650.024.574519
GCGAATC10300.024.45142233
AATCTCG8950.023.51354636
TATGCCG8700.022.99996643
CGACGAG3800.022.69696424
ACGCAGA75500.022.481357
CGCAGAG77000.021.9537948
TCTCGTA8400.021.76746738
TCGACGA2700.021.72254851
CGAATCT10100.021.51977234
CTCGTAT8600.021.26194839
ACTAGCG4300.020.86077955
Produced by FastQC (version 0.11.5)