FastQCFastQC Report
Sat 18 Jun 2016
SRR3137997_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137997_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3550514
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG95070.26776404768436346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33050.039.4942781
GTATCAA65450.034.256091
ATCAACG80050.027.4989593
CAACGCA82250.027.108055
TCAACGC82800.026.7558424
TATCAAC82950.026.696912
AACGCAG86800.025.848796
TGCGACG3500.023.6538822
ACGCAGA100300.022.0669237
CGCAGAG102450.021.4707438
CAATGCG3950.020.95676419
AGAGTAC104400.020.11767611
GTGTAGC20100.019.7046281
CTCGATA5550.019.28068565
GTACATG95200.019.0651441
GCTCGAT5450.018.9994864
CAGAGTA112650.018.97896210
CGACGAG4400.018.81691424
CCGATCG5000.018.6195379
GAGTACT70950.018.56934412
Produced by FastQC (version 0.11.5)