FastQCFastQC Report
Sat 18 Jun 2016
SRR3137997_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137997_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3550514
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32150.038.2196121
GTATCAA62600.033.357871
ATCAACG74650.027.3143
CAACGCA75800.026.9447385
TATGCCG6850.026.69320343
TCAACGC76750.026.566274
GCTCGAT5150.026.12672464
TATCAAC79900.025.7358972
AACGCAG80550.025.5692526
CTCGTAT7200.023.95880139
TCGTATG7650.023.45144340
CTCGATA5600.023.41117365
TCTCGTA7300.022.68569638
GCGAATC9800.022.17869433
ACGCAGA93200.021.7643767
AATCTCG7950.021.26456836
CGCAGAG96300.020.9562878
TGGTCGT6650.020.23347951
AGAGTAC99550.019.75233311
TAAGGCG13100.019.75201829
Produced by FastQC (version 0.11.5)