FastQCFastQC Report
Sat 18 Jun 2016
SRR3137996_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137996_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3338433
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG108670.3255119991924355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31550.038.8561551
GTATCAA68800.034.9362451
ATCAACG81200.028.8888743
CAACGCA84150.028.1770445
TGCGACG5300.027.98627522
TCAACGC83750.027.8938334
CGACGAG5350.027.72555524
TATCAAC85650.027.622192
AACGCAG87600.027.4259416
ATGCGAC5950.027.24587821
CAATGCG5600.025.86792419
GCTCGAT5950.024.36348564
ACGCAGA98750.023.81037
CTCGATA6250.023.19403865
CGCAGAG100050.023.1577268
GCGACGA6550.022.64470123
AATGCGA7000.022.1731720
AGAGTAC100550.021.57265511
GTACTAG9650.021.0539671
GAGTACT61600.020.99385312
Produced by FastQC (version 0.11.5)