FastQCFastQC Report
Sat 18 Jun 2016
SRR3137996_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137996_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3338433
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31050.039.9036751
GTATCAA61600.034.1205941
TGCGACG4850.030.58613422
CTCGATA6600.028.22716365
GCTCGAT6600.028.22716364
ATCAACG76500.026.6983383
TATCAAC79150.026.1535572
CAACGCA78650.026.0123675
CGCACTA5450.025.95402758
CGACGAG5850.025.9478424
TCAACGC79400.025.766664
AACGCAG82600.024.9765246
ATGCGAC6150.024.6817221
GCGCGAT3100.024.48267613
CGTACGA3950.024.45596760
TATGCCG7200.024.43776943
TGGTCGT7850.023.29360251
TCGTCTA8150.023.28316354
CGATAAT8350.023.13798167
CAATGCG6450.022.99887719
Produced by FastQC (version 0.11.5)