FastQCFastQC Report
Sat 18 Jun 2016
SRR3137995_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137995_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3163623
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG116390.3679009793518381No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25400.041.224231
GTATCAA55350.034.723961
ATCAACG66350.028.3988253
CAACGCA68750.027.6188015
TCAACGC69650.027.1586634
TATCAAC70900.026.7664382
AACGCAG73250.026.3042816
GCTCGAT3500.025.63969864
TGCGACG2450.022.5282422
CTCGATA4150.022.45517365
ACGCAGA83650.022.4226027
CGCAGAG85700.021.9282078
GAGTACT61300.020.08591512
AGAGTAC90350.019.65906711
GTACATG80100.019.264521
CGACGAG2901.8189894E-1219.03278224
TACATGG77200.018.960812
CAGAGTA95750.018.83732610
GCAGAGT98600.018.6749179
ACGATCG1852.6412272E-718.65230660
Produced by FastQC (version 0.11.5)