FastQCFastQC Report
Sat 18 Jun 2016
SRR3137995_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137995_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3163623
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26150.039.5993231
GTATCAA58200.035.5256541
AATCGCG1800.032.58328636
TGCGACG2700.031.9423822
ATCAACG68450.028.9310233
TATCAAC71900.028.1190682
TCAACGC70850.028.0483934
CAACGCA70350.028.0025375
AACGCAG75050.026.7537046
CGAATCG2550.025.70584734
ATGCGAC4150.024.10687621
CGACGAG3500.023.65598924
GCGAATC12400.023.09307533
ACGCAGA84150.023.0402377
CAATGCG3900.022.99851419
CGCAGAG86050.022.411238
AATCTCG9600.022.28122136
TATGCCG9100.021.6098643
TAAGGCG15750.021.24792529
TCGTATG9300.021.14513240
Produced by FastQC (version 0.11.5)