FastQCFastQC Report
Sat 18 Jun 2016
SRR3137994_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137994_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4941690
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG212090.42918515730448487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41650.037.7855871
GTATCAA82800.033.3556181
ATCAACG96300.028.0086733
CAACGCA98050.027.764835
TCAACGC99800.027.1691514
TATCAAC104100.026.1027622
AACGCAG106050.025.7400466
TGCGACG6350.024.9874922
CAATGCG6600.024.55963519
CGACGAG6950.024.31997124
CTCGATA6200.023.94169465
ATGCGAC7300.022.20572921
ACGCAGA119800.022.18637
CGCAGAG122100.021.6000678
GCTCGAT6900.021.51195164
CGTGCGC8700.021.40156610
ACGTGCG3300.019.8496769
AGAGTAC126750.019.78007711
GTGTAGC24950.019.323351
GAGTACT80750.018.96248212
Produced by FastQC (version 0.11.5)