FastQCFastQC Report
Sat 18 Jun 2016
SRR3137994_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137994_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4941690
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG54950.11119677681117189RNA PCR Primer, Index 39 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39950.034.9050981
GTATCAA79250.033.928331
CTCGTAT21900.028.986539
TATGCCG22900.028.77530943
ATCAACG91300.028.0768833
CAACGCA92650.027.5930215
TCGTATG24350.027.34515840
TCTCGTA23400.026.98095338
ATCTCGT23550.026.95559937
ATGCCGT25500.026.24724244
TATCAAC99750.026.2177832
TCAACGC98100.026.0952474
AACGCAG99950.026.0949636
TGCCGTC27300.024.3902845
CCGTCTT27850.023.53696847
GCCGTCT28400.022.95966346
CGTATGC29250.022.52858741
ACGCAGA113100.022.2972077
CGCAGAG116250.021.5446478
CGTCTTC30550.021.45677848
Produced by FastQC (version 0.11.5)