FastQCFastQC Report
Sat 18 Jun 2016
SRR3137993_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137993_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4158252
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG160260.3854023277088546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40250.039.1962931
GTATCAA80450.032.4127041
GCTCGAT7400.030.32135464
CGACGAG5100.028.40604424
CTCGATA8000.028.04860965
TGCGACG5600.027.10233922
ATCAACG96300.026.366953
TGGTCGT8750.025.63856351
CAACGCA100350.025.484915
TCAACGC101400.025.1472724
TATCAAC103600.024.7035182
CAATGCG6150.024.67736219
AACGCAG106600.024.3819436
GTCGTCT9650.023.96212653
CGATAAT9750.023.7226667
TCAGCGC10750.023.43986944
GCTCGTC10400.023.22544139
TCGTCTA10900.022.16326354
CCGTCGG4850.022.04710624
CGTCATT11550.021.80185526
Produced by FastQC (version 0.11.5)