FastQCFastQC Report
Sat 18 Jun 2016
SRR3137993_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137993_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4158252
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41200.038.618811
GTATCAA79450.033.5365641
CGACGAG5850.032.43383424
CAATGCG6300.031.21230119
TATGCCG14350.028.36958143
TGCGACG6750.028.10966122
CTCGTAT14450.027.69640239
TCGTATG15000.027.14088440
ATCAACG96950.027.0104893
GCTCGAT7800.026.98067764
CTCGATA7850.026.80914565
CAACGCA99000.026.3814815
ATGCGAC8500.026.38099121
TCAACGC101450.025.8123874
TATCAAC103350.025.7715232
ATCTCGT15500.025.59668537
AACGCAG106550.024.7699746
ATGCCGT16300.024.76401544
GCTCGTC9000.024.53413239
TGGTCGT9150.024.50928951
Produced by FastQC (version 0.11.5)