FastQCFastQC Report
Sat 18 Jun 2016
SRR3137992_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137992_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5145367
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG230100.4471984214148379No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43300.036.987091
GTATCAA83650.032.856561
CAACGCA100300.027.2502355
ATCAACG99500.027.109473
TCAACGC101600.026.693344
AACGCAG106650.026.0839966
TATCAAC105450.025.604022
TGCGACG7250.023.78880922
CGACGAG7450.022.68828824
ACGCAGA123150.021.9776547
CGCAGAG126250.021.2758058
CAATGCG8150.021.15997119
GCTCGAT7900.020.53542364
AGAGTAC127850.020.06833611
GCGACGA9000.019.16376923
GAGTACT77800.019.0162712
CAGAGTA138300.018.94982510
GTACATG128450.018.7961561
CGCACTA8150.018.63094158
GCAGAGT144050.018.2398669
Produced by FastQC (version 0.11.5)