FastQCFastQC Report
Sat 18 Jun 2016
SRR3137992_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137992_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5145367
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG62420.12131301809958357RNA PCR Primer, Index 39 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39750.038.378051
GTATCAA80450.033.5060161
TATGCCG23750.030.94145643
CTCGTAT23850.029.79912839
ATCTCGT25350.028.03559337
TCTCGTA25250.028.01053638
TCGTATG26150.027.9697740
ATCAACG95300.027.4772723
CAACGCA96100.027.3551715
TCAACGC101050.026.117584
ATGCCGT27950.026.04506144
TATCAAC102500.026.019652
CGTATGC28600.025.69438741
AACGCAG103650.025.6283596
TGCCGTC28500.025.54218945
TGCGACG7000.025.13481722
GCCGTCT29400.024.9949846
CCGTCTT29450.024.95254347
CGTACGA4550.024.26428660
CGACGAG7700.023.298124
Produced by FastQC (version 0.11.5)