FastQCFastQC Report
Sat 18 Jun 2016
SRR3137991_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137991_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4543773
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG222040.4886687781277806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37300.034.90351
GTATCAA72350.034.0371741
CAACGCA85700.028.631795
ATCAACG84600.028.5042363
TCAACGC87450.027.6977374
AACGCAG92100.027.0205356
TATCAAC91550.026.7086832
ACGCAGA103650.023.5143817
CGCAGAG104250.023.0506558
GCTCGAT6650.022.31910764
TGCGACG4050.022.14325122
GAGTACT65250.020.50723812
AGAGTAC108150.020.43940711
CAGAGTA114800.019.67358410
CTCGATA7600.019.53029665
GTACATG111050.019.1980931
AGTACTT70500.019.12896713
TACATGG109250.019.1030922
ACGTGCG3450.018.9880479
CGACGAG4750.018.88066124
Produced by FastQC (version 0.11.5)