FastQCFastQC Report
Sat 18 Jun 2016
SRR3137991_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137991_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4543773
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG58700.12918779173167322RNA PCR Primer, Index 39 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32850.035.9340321
GTATCAA66450.034.437671
ATCAACG78800.027.9783783
CAACGCA79700.027.7054235
TATGCCG23100.027.18152643
TCAACGC82350.026.8557664
TATCAAC84550.026.4437892
AACGCAG87000.025.7770046
TCGTATG25700.024.16316640
CTCGTAT23350.023.78775639
TCTCGTA23100.023.74649838
CGACGAG5600.022.79440124
TGCGACG5450.022.78874822
CGTATGC27650.022.45907241
ATGCCGT27950.022.34144244
ACGCAGA99100.022.0012977
ATCTCGT25600.021.83179937
TGCCGTC28550.021.63024145
GCGCGAT2900.021.41285913
CCGTCTT28700.021.27677547
Produced by FastQC (version 0.11.5)