FastQCFastQC Report
Sat 18 Jun 2016
SRR3137990_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137990_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5029209
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG170290.3386019550987044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43200.040.2701151
GTATCAA88500.034.371041
ATCAACG101850.029.2582023
TCAACGC105850.028.189594
CAACGCA106350.028.1277395
TATCAAC111300.027.204642
AACGCAG111700.027.1247776
TGCGACG4250.024.34838322
ACGCAGA128900.023.0036747
CGCAGAG130850.022.4505068
AGAGTAC135300.020.59493611
CTCGATA7600.020.43909365
CGATAAT7700.019.7253567
CAGAGTA147400.019.37107810
GCTCGAT7850.019.34804364
GCAGAGT150600.019.2098189
TAGGTCG7800.019.01491421
GCGCGAT3450.018.99398213
GAGTACT86550.018.88716712
TACATGG119800.018.8623732
Produced by FastQC (version 0.11.5)