FastQCFastQC Report
Sat 18 Jun 2016
SRR3137990_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137990_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5029209
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41750.041.0855521
GTATCAA83900.032.7857861
TATGCCG16800.026.69653543
ATCAACG100750.026.57363
CAACGCA102200.026.2975945
TCAACGC103150.025.9884994
TATCAAC105500.025.8028052
CTCGTAT17400.024.98285739
AACGCAG108050.024.9698376
TCGTATG19100.024.02365340
TCTCGTA18300.023.56566638
CGTATGC20250.022.31860541
ATGCCGT20750.021.94707544
TGCCGTC21700.021.62220645
ATCTCGT20200.021.178337
CCGTCTT22100.021.07474347
ACGCAGA128250.020.6856027
GCCGTCT22500.020.54674546
CGCAGAG130450.020.2838548
TGCGACG4750.019.61001822
Produced by FastQC (version 0.11.5)