FastQCFastQC Report
Sat 18 Jun 2016
SRR3137989_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137989_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3778210
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG118930.31477869149676696No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36500.041.336771
GTATCAA72200.033.588511
CAACGCA91550.026.426255
ATCAACG90150.026.405973
GCTCGAT5800.026.18623264
TGCGACG5200.025.86942322
TCAACGC93600.025.4742054
AACGCAG97150.025.1559546
TATCAAC95500.025.1394582
CTCGATA6150.024.1372565
CGTACGA3750.023.92627160
TCGTCTA7200.022.53062854
ACGCAGA111100.021.6586117
ATGCGAC7100.020.88764421
CGACGAG6450.020.85652224
CGCAGAG115150.020.6279478
CGATAAT7450.020.38957467
GTACATG117450.020.2665711
TACATGG119400.019.4149932
GTACTAG10250.019.1560631
Produced by FastQC (version 0.11.5)