FastQCFastQC Report
Sat 18 Jun 2016
SRR3137989_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137989_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3778210
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37150.038.8364831
GTATCAA76300.034.3804441
ATCAACG92500.027.712393
CAACGCA93100.027.570855
TCAACGC94650.027.22874
TATCAAC96500.026.7782022
TATGCCG12450.026.60324343
AACGCAG98800.026.153446
GCTCGAT7200.025.8747864
CTCGTAT12350.025.42151339
TCGTATG13500.024.78966340
TGGTCGT7850.024.61223451
CTCGATA7500.024.3794765
TGCGACG5550.024.24335922
CGATAAT7850.023.73322767
TCTCGTA13350.023.51696638
CGACGAG5800.023.19869424
GCTCGTC8650.023.13336839
ACGCAGA112250.022.7108447
GTCGTCT8700.022.2075953
Produced by FastQC (version 0.11.5)