FastQCFastQC Report
Sat 18 Jun 2016
SRR3137988_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137988_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3568820
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG110810.3104947853912498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30650.037.6393051
GTATCAA60200.032.6638151
TGCGACG3700.028.9010122
ATCAACG71100.026.9869043
CAACGCA73100.026.3034275
TCAACGC73900.026.1556284
TATCAAC74800.026.0109312
AACGCAG76650.025.4505626
ATGCGAC4800.022.99262821
CAATGCG5000.022.7636619
CGACGAG4700.022.752524
ACGCAGA89500.021.3794867
GCGACGA5050.021.17523
CGCAGAG92600.020.5895828
CGGCGAT1851.1805241E-820.51039523
TGGTCGT5000.020.01877651
GTGTAGC20250.019.8970241
GCTCGAT5500.019.45562664
AGAGTAC93450.019.40889711
GAGTACT60550.019.24904312
Produced by FastQC (version 0.11.5)